Month: December 2018

What is gene editing and CRISPR-CAS9?

  Altering the DNA or gene sequences present into the genome of an organism is called as a gene editing. The alteration may be addition or deletion. CRISPR-CAS9 is the system used as a gene editing tool in recent days.   In simple words, we can describe the gene editing as editing the messages of

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How to prepare lysis buffer for different types of DNA extraction methods?

A crucial step in genomic DNA extraction in any DNA extraction protocol is to lyse the cell. Breaking the cell wall or nuclear membrane with the help of different types of chemical, physical and enzymatic method is called as lysis or cell lysis.   In this article, we are discussing different types of cell lysis

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Importance of Tris-EDTA (TE) buffer in DNA extraction

Tris and EDTA are two major components which are used throughout the DNA extraction protocol. Tris and EDTA are applicable in lysis buffer preparation, elution buffer preparation and washing buffer preparation. The major role of TE buffer in DNA extraction is to dissolve DNA into liquid form. However, it is also used as lysis buffer.

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