PCR primer design guideline: PCR primers are similar as like primer involved in DNA replication in vivo but the major difference between both types of primers are, PCR primers are DNA primers while the in vivo replication primers are RNA primers. In this article, we are discussing the PCR primer and their properties along with
Linking number is a topological property of DNA. Linking number is a sum of twists and writhes. The numbers of time one strand of DNA turned around another strand is called as a twist while inter-coiling of the double helix is termed as a writhe. In short, writhe is a number of a time
Chromosomal aberrations, point mutation, deletion of nucleotides and addition of nucleotides, loss of function and gain of function are some of the examples of different type of Genetic mutations. The term mutation was coined by Hugo De Vries in 1890. Mutation is an important phenomenon in nature for the creation of variation. “Under the influence of