A crucial step in genomic DNA extraction in any DNA extraction protocol is to lyse the cell. Breaking the cell wall or nuclear membrane with the help of different types of chemical, physical and enzymatic method is called as lysis or cell lysis. In this article, we are discussing different types of cell lysis
Tris and EDTA are two major components which are used throughout the DNA extraction protocol. Tris and EDTA are applicable in lysis buffer preparation, elution buffer preparation and washing buffer preparation. The major role of TE buffer in DNA extraction is to dissolve DNA into liquid form. However, it is also used as lysis buffer.
Different types of nucleic acid purification methods are available for different types of tissue. Alcohol purification, silica gel column based purification, agarose gel-based DNA purification, automated nucleic acid purification and chemical based nucleic acid purification methods are commonly used in genetic laboratories. In fact, the nucleic acid purification is followed by the extraction method or