Category: Educational articles

DNA gel loading dye

DNA gel loading dye composition, preparation and function. Loading dye is an important component in agarose gel electrophoresis. The loading dye contains bromophenol blue, Ficoll 400 and water majorly while Xylene cyanol, Tris and EDTA are optional. Bromophenol blue is one of the most popular indicators of DNA in agarose gel electrophoresis. Bromophenol blue is

Read More

Role of EtBr in molecular genetics and cytogenetics

Role of Ethidium bromide in agarose gel electrophoresis and Cytogenetics The role of EtBr in agarose electrophoresis is to intercalate with DNA and helps in the identification of DNA fragments. Yet, it plays a significant role in cytogenetics as well. In this article, We will discuss the role of EtBr in molecular genetics and cytogenetics. Ethidium

Read More

Different types of Genetic mutations

Chromosomal aberrations, point mutation, deletion of nucleotides and addition of nucleotides, loss of function and gain of function are some of the examples of different type of Genetic mutations. The term mutation was coined by Hugo De Vries in 1890. Mutation is an important phenomenon in nature for the creation of variation.   “Under the influence

Read More