Prokaryotic DNA replication: Replication class 2

How does replication occur in prokaryotes? In 1963, J Cairns┬áreported the process of replication in E.coli bacteria by autoradiography. Replication is a process in which…

Role of MgCl2 in PCR reaction

The function of MgCl2 in PCR reaction. Polymerase chain reaction is one of the important experimental procedure in downstream genomic research. It generates millions of…

DNA Topology class 2: Linking number and supercoiling

What is linking number and how it is important for supercoiling?   Linking number is a topological property of DNA. Linking number is a sum…