Tag: DNA

Prokaryotic DNA replication: Replication class 2

Team Genetic Education 08/08/2018 No Comments All articles, Replication , , , , , , , , , , ,

How does replication occur in prokaryotes? In 1963, J Cairns reported the process of replication in E.coli bacteria by autoradiography. Replication is a process in which a DNA molecule is copied. From the double-stranded DNA, two daughter dsDNA is generated and is transferred to two different daughter cells. Before proceeding to this article please read the […]

Role of MgCl2 in PCR reaction

Team Genetic Education 06/08/2018 1 Comment All articles, PCR technology , , , , ,

The Role of MgCl2 in PCR reaction is to enhance the DNA amplification by boosting the activity of Taq DNA polymerase. Polymerase chain reaction is one of the important experimental procedure in downstream genomic research. It generates millions of amplicon for the gene of our interest. The process is as similar to replication but it […]

DNA Topology class 1: Topoisomerase

Team Genetic Education 31/07/2018 2 Comments All articles, DNA topology , , , , , , , , ,

Role of Topoisomerase I and Topoisomerase II in DNA topology Topoisomerase is a class of enzyme which helps in winding and unwinding of DNA. Three forms of DNA is most prevalent in nature: circular, linear and supercoiled. Circular DNA is covalently closed and does not have any interruption in between nucleotides. Circular DNA is found in […]

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