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Tag: DNA

Prokaryotic DNA replication: Replication class 2

How does replication occur in prokaryotes? In 1963, J Cairns reported the process of replication in E.coli bacteria by autoradiography. Replication is a process in which a DNA molecule is copied. From thedouble-stranded DNA, two daughter dsDNA is generated and is transferred to two different daughter cells. Before proceeding to this article please read the General

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Role of MgCl2 in PCR reaction

The Role of MgCl2 in PCR reaction is to enhance the DNA amplification by boosting the activity of Taq DNA polymerase. Polymerase chain reaction is one of the important experimental procedure in downstream genomic research. It generates millions of amplicon for the gene of our interest. The process is as similar to replication but it

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DNA Topology class 1: Topoisomerase

Role of Topoisomerase I and Topoisomerase II in DNA topology Topoisomerase is a class of enzyme which helps in winding and unwinding of DNA. Three forms of DNA is most prevalent in nature: circular, linear and supercoiled. Circular DNA is covalently closed and does not have any interruption in between nucleotides. Circular DNA is found in

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