PCR stands for Polymerase chain reaction. It is a laboratory genetic technique used to amplify DNA and generate copies for downstream analysis. Learn PCR, its principle, steps, advanced protocols, troubleshooting, types and other related information in this category.
Explore all articles on PCR technology and master this essential molecular skill.
“RT-PCR can effectively quantify copies of a gene present in the sample. Thus, it’s widely used in gene expression studies, cancer research and viral load estimation.” PCR (Polymerase Chain Reaction) […]
“Real-time PCR is a technique used for the quantification of nucleic acid present in the sample using either fluorescence dye or probe-based technique.” PCR is capable enough to amplify the
Real-time PCR: Principle, Procedure, Advantages, Limitations and Applications Read More »
“Inverse PCR is used for site-directed mutagenesis and insert studies. Learn about the technique, concept, protocol and applications of inverse PCR.” PCR (Polymerase Chain Reaction) is a technique to amplify
Inverse PCR: Principle, Procedure, Protocol and Applications Read More »
“PCR inhibitors are biological, chemical or other agents that negatively affect the PCR reaction. In this article, learn about various types of PCR inhibitors, the mechanism of inhibition, and how
What are PCR Inhibitors and How to Remove Them? Read More »
“Explore 7 key roles of PCR in DNA sequencing— beyond just amplification! Learn how PCR is applied in sequencing in this insightful article. If someone asks this question, the straightforward
Why is PCR used in the Process of DNA Sequencing? Read More »
“Long-range PCR is a method to amplify the DNA fragment of more than 5Kb using a special type of high-fidelity DNA polymerase.” Using the PCR ingredients like dNTPs, PCR buffer,
What is a Long-Range PCR? Read More »
