qPCR acronym as quantitative PCR is a technique to measure the amount of DNA present in a sample. It can study gene expression by utilizing either the TaqMan probe or …
Easiest qPCR Protocol using TaqMan Probe and SYBR Green Dye Read More »
An exclusive native PCR modification known as Asymmetric PCR has been applied to amplify only a single strand during the PCR. PCR is a thermocycler, amplifies DNA. Meaning, it helps …
Asymmetric PCR: Principle, Process, Protocol, Applications, Advantages and Limitations Read More »
“The percent of Guanine and Cytosine, nitrogenous bases present in a DNA, RNA, nucleic acid sequence or primer is known as GC content. The unit to calculate the GC content …
Positive, Negative and Internal controls are common PCR control types employed as a reference to evaluate results. If you know PCR well, you probably understand why we use the controls! …
Type of PCR Controls- Negative, Positive and Internal Controls Read More »
The annealing temperature is the temperature at which the PCR primers bind to the complementary template region, usually is between 50ºC to 68ºC. We have started exploring PCR, PCR optimization …
What is Annealing Temperature in PCR? How to Calculate and Set It? Read More »
Usually, 25 to 35 cycles a typical PCR reaction needs to get a good amount of amplification, containing denaturation, annealing and extension simultaneously at 95ºC, 50 to 68ºC and 72ºC. …
