Comprehensive Guide to Plasmid DNA Isolation
“Plasmid DNA isolation is a crucial scheme in genetic engineering and gene therapy. Let’s understand the process of plasmid DNA isolation along with one standard protocol.” Plasmids are small, circular …
Genetics vs Epigenetics: From Gene Alterations to Gene Expression
“Genetics usually comprises the study of genes and related alterations while epigenetics comprises the study of gene expression and proteins involved in the process. Let’s check out a few important …
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Sanger Sequencing vs PCR: Common and Technical Differences
“Sanger sequencing is a genetic technique to determine the order of nucleotides while PCR generates copies of the DNA fragment, present in the sample. Let’s see some of the significant …
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Common Issues in DNA/RNA Gel Electrophoresis and Troubleshooting
“Common problems in gel electrophoresis are the absence of bands, band smears, weak resolution, diffused bands, wavy bands and poor band separation. This article comprises common troubleshooting to overcome these …
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How to Extract DNA from Bacteria? + Protocol
“Extracting DNA from bacteria is a relatively easy technique due to their simple structure. Methods like CTAB, PCI and even ready-to-use kits give excellent results. However, to get good results …
7 Ways to Determine Genotypes Using Gel Electrophoresis
“Native gel electrophoresis helps in genetic screening and diagnosis by determining genotypes and identifying homozygous and heterozygous. This article explains 7 ways that this technique can be used for genotyping.” …
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