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DNA DNA sequencing Genetic Education

What is the Role of ddNTPs? 

Dr Tushar Chauhan 21/09/2020 0 Comments

The dideoxynucleotides are known as the chain terminator nucleotides that abort the nucleotide chain due to lack of 3’ OH group.  Within the past 20 to 25 years the DNA…

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DNA DNA sequencing Genetic Education Miscellaneous

DNA fragmentation- Techniques, Importance and Applications

Dr Tushar Chauhan 17/08/2020 0 Comments

“DNA fragmentation is a technique of artificial breaking of DNA in order to use it in various downstream genetics and well as genomics applications.”  The first DNA fragmentation was reported…

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DNA sequencing Genetic Education

Why is mRNA chosen for making DNA Library instead of Genomic DNA? 

Dr Tushar Chauhan 17/08/2020 0 Comments

“The mRNA possesses the sequences to encode protein only thus to construct the DNA library or the cDNA library, the mRNA is reverse transcribed into the cDNA and processed.”  The…

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DNA DNA sequencing Genetic Education

What is Whole-Exome Sequencing? 

Dr Tushar Chauhan 27/07/2020 0 Comments

“Determining the nucleotide sequence orders of the whole exome set of an organism using a high throughput DNA sequencing method is known as whole-exome sequencing.”  Three different biomolecules construct a…

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DNA sequencing Genetic Education PCR technology

16S rRNA: Gene, Sequencing and Importance

Dr Tushar Chauhan 18/05/2020 0 Comments

“The 16S rRNA gene is a bacterial ribosomal gene and a part of the 30S subunit which is used in the identification, characterization, and classification of various bacteria.” 16S rRNA…

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DNA sequencing Genetic Education

Preparing “DNA for Sequencing”

Dr Tushar Chauhan 27/04/2020 0 Comments

“Preparing ‘DNA for sequencing’ is a crucial and important step in the DNA sequencing process. The quality and quantity of the template DNA are two key factors for getting better…

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