What is the Role of ddNTPs?
The dideoxynucleotides are known as the chain terminator nucleotides that abort the nucleotide chain due to lack of 3’ OH group. Within the past 20 to 25 years the DNA…
Continue readingDNA fragmentation- Techniques, Importance and Applications
“DNA fragmentation is a technique of artificial breaking of DNA in order to use it in various downstream genetics and well as genomics applications.” The first DNA fragmentation was reported…
Continue readingWhy is mRNA chosen for making DNA Library instead of Genomic DNA?
“The mRNA possesses the sequences to encode protein only thus to construct the DNA library or the cDNA library, the mRNA is reverse transcribed into the cDNA and processed.” The…
Continue readingWhat is Whole-Exome Sequencing?
“Determining the nucleotide sequence orders of the whole exome set of an organism using a high throughput DNA sequencing method is known as whole-exome sequencing.” Three different biomolecules construct a…
Continue reading16S rRNA: Gene, Sequencing and Importance
“The 16S rRNA gene is a bacterial ribosomal gene and a part of the 30S subunit which is used in the identification, characterization, and classification of various bacteria.” 16S rRNA…
Continue readingPreparing “DNA for Sequencing”
“Preparing ‘DNA for sequencing’ is a crucial and important step in the DNA sequencing process. The quality and quantity of the template DNA are two key factors for getting better…
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