Differences between Genotyping vs Sequencing
Genotyping is a process of investigating a variant(s) present in an individual while sequencing is a process of investigating the whole sequence, gene or genome. Genotyping and sequencing are separate …
DNA sequencing
The Concept of ChIP-Seq (ChIP-Sequencing) Explained
“Sequence information of DNA linked with associated proteins like histones can be obtained by performing high throughput DNA sequencing, such technique is known as ChIP-Seq.” Highlights: ChIP-seq was developed by …
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What is the Role of ddNTPs?
The dideoxynucleotides are known as the chain terminator nucleotides that abort the nucleotide chain due to lack of 3’ OH group. Within the past 20 to 25 years the DNA …
DNA fragmentation- Techniques, Importance and Applications
“DNA fragmentation is a technique of artificial breaking of DNA in order to use it in various downstream genetics and well as genomics applications.” The first DNA fragmentation was reported …
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Why is mRNA chosen for making DNA Library instead of Genomic DNA?
“The mRNA possesses the sequences to encode protein only thus to construct the DNA library or the cDNA library, the mRNA is reverse transcribed into the cDNA and processed.” The …
Why is mRNA chosen for making DNA Library instead of Genomic DNA? Read More »
What is Whole-Exome Sequencing?
“Determining the nucleotide sequence orders of the whole exome set of an organism using a high throughput DNA sequencing method is known as whole-exome sequencing.” Three different biomolecules construct a …
