“Parameters that influence the Sanger sequencing results are good quality extract, amplicon purification, primer designing and managing leftover ddNPTs. These parameters can improve the Sanger Sequencing results, certainly.” Let’s findout …
How to improve Sanger Sequencing Results?- 5 Technical Tips from Experts Read More »
“A sequencing read is referred to as nucleotides of a single DNA fragment from a library. Sequencing reads, read lengths, numbers, read alignments and read scores are important terminologies, technically. …
“A computational/graphical representation of nucleic acid separation generated during high throughput electrophoresis is referred to as an electropherogram.” This article mainly focuses on what is an electropherogram of DNA or …
Genotyping is a process of investigating a variant(s) present in an individual while sequencing is a process of investigating the whole sequence, gene or genome. Genotyping and sequencing are separate …
“Sequence information of DNA linked with associated proteins like histones can be obtained by performing high throughput DNA sequencing, such technique is known as ChIP-Seq.” Highlights: ChIP-seq was developed by …
The Concept of ChIP-Seq (ChIP-Sequencing) Explained Read More »
The dideoxynucleotides are known as the chain terminator nucleotides that abort the nucleotide chain due to lack of 3’ OH group. Within the past 20 to 25 years the DNA …
