How to Isolate mRNA from Total Cellular RNA?
“Only 5% of total cellular RNA content is mRNA and is isolated by removing all other non-coding RNAs from the sample. The unique 3’-OH end of mRNA is utilized to …
“Only 5% of total cellular RNA content is mRNA and is isolated by removing all other non-coding RNAs from the sample. The unique 3’-OH end of mRNA is utilized to …
A monophasic solution of guanidinium isothiocyanate and phenol is known as Trizol or Tri-reagent provides stability and integrity to RNA during isolation. Commercially, from company to company the name varies …
Effectual Trizol RNA Extraction Protocol using Tri Reagent Read More »
“DEPC (Diethyl pyrocarbonate) is a nuclease inhibitory agent, used in the RNA isolation to inactivate the RNase enzyme.” The single-stranded structure of the RNA makes it more degradative than DNA, …
11 Effective Ways to Use DEPC-Treated Water in RNA Isolation Read More »
“RNA extraction is a process to isolate various or a specific type of RNA molecules for gene expression studies.” Snippet: The function of RNase is to cleave RNA. It is …
RNA Extraction: Principle, Procedure, Protocol and Importance Read More »
“Microsatellite instability is defined as indel (insertion or deletion) mutations within the repetitive regions (microsatellite) in cancer cells by the inactivation of DNA mismatch repair proteins.“ Do you know? Introns …
Role of Microsatellite Instability (MSI) in cancer (MSI-H, MSI-I and MSS) Read More »
“Loss of Heterozygosity (LOH) is defined as the absence of one normal parental allele or gene (or part of the chromosome) in an organism which usually results in cancer.“ Like …
What is Loss of Heterozygosity? How It is Associated with Cancer? Read More »