How to Isolate mRNA from Total Cellular RNA?
“Only 5% of total cellular RNA content is mRNA and is isolated by removing all other non-coding RNAs from the sample. The unique 3’-OH end of mRNA is utilized to …
Genetic Education
Effectual Trizol RNA Extraction Protocol using Tri Reagent
A monophasic solution of guanidinium isothiocyanate and phenol is known as Trizol or Tri-reagent provides stability and integrity to RNA during isolation. Commercially, from company to company the name varies …
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11 Effective Ways to Use DEPC-Treated Water in RNA Isolation
“DEPC (Diethyl pyrocarbonate) is a nuclease inhibitory agent, used in the RNA isolation to inactivate the RNase enzyme.” The single-stranded structure of the RNA makes it more degradative than DNA, …
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RNA Extraction: Principle, Procedure, Protocol and Importance
“RNA extraction is a process to isolate various or a specific type of RNA molecules for gene expression studies.” Snippet: The function of RNase is to cleave RNA. It is …
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Role of Microsatellite Instability (MSI) in cancer (MSI-H, MSI-I and MSS)
“Microsatellite instability is defined as indel (insertion or deletion) mutations within the repetitive regions (microsatellite) in cancer cells by the inactivation of DNA mismatch repair proteins.“ Do you know? Introns …
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What is Loss of Heterozygosity? How It is Associated with Cancer?
“Loss of Heterozygosity (LOH) is defined as the absence of one normal parental allele or gene (or part of the chromosome) in an organism which usually results in cancer.“ Like …
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