“PAM stands for Protospacer Adjacent Motif, which functions as two-factor authentication for Cas to cleave the foreign invading DNA only.” The CRISPR system has been an integral part of bacterial …
Importance of PAM Sequence (Protospacer Adjacent Motif) in CRISPR System Read More »
“CRISPR naturally has spacer sequences in-between and works in sync with the CAS protein as an adaptive immune system in bacterial and archaea.” The story of CRISPR and its fascinating …
How does the CRISPR-Mediated Adaptive Immune System Work in Bacteria? Read More »
CRISPRi or CRISPR activation is a process to upregulate gene expression or activate genes by effector activators, dcas9 and sgRNA. In 2021, CRISPR have deep penetration in diverse scientific fields. …
“PCR amplification is an enzyme-dependent process to copy a DNA sequence, DNA fragment, genomic DNA, plasmid DNA or any nucleic acid.“ In other words, we can say, Producing multiple copies …
10 Strategies to Achieve Excellent PCR Amplification Read More »
CRISPRi, short for CRISPR interference, is designed to repress gene expression using the customized sgRNA and dCAS. CRISPR- CAS9 is simply amazing! Why? Because it can edit a gene at …
“dCAS9 is a deactivated or dead Cas protein that lacks nucleophilic activity or simply is a deactivated nuclease CAS9.” You may wonder, Without nucleophilic catalytic activity, what is the use …
dCAS9 (Dead CAS system): Concept, Functions and Applications Read More »
