How to Isolate mRNA from Total Cellular RNA?
“Only 5% of total cellular RNA content is mRNA and is isolated by removing all other non-coding RNAs from the sample. The unique 3’-OH end of mRNA is utilized to …
Effectual Trizol RNA Extraction Protocol using Tri Reagent
A monophasic solution of guanidinium isothiocyanate and phenol is known as Trizol or Tri-reagent provides stability and integrity to RNA during isolation. Commercially, from company to company the name varies …
Effectual Trizol RNA Extraction Protocol using Tri Reagent Read More »
11 Effective Ways to Use DEPC-Treated Water in RNA Isolation
“DEPC (Diethyl pyrocarbonate) is a nuclease inhibitory agent, used in the RNA isolation to inactivate the RNase enzyme.” The single-stranded structure of the RNA makes it more degradative than DNA, …
11 Effective Ways to Use DEPC-Treated Water in RNA Isolation Read More »
21 Things to know for Effective RNA Extraction
“RNA extraction is a process to isolate RNA from a cell for gene expression studies and pathogen identification.” RNA extraction is a nucleic acid separation technique to collect various types …
RNA Extraction: Principle, Procedure, Protocol and Importance
“RNA extraction is a process to isolate various or a specific type of RNA molecules for gene expression studies.” Snippet: The function of RNase is to cleave RNA. It is …
RNA Extraction: Principle, Procedure, Protocol and Importance Read More »
Role of Microsatellite Instability (MSI) in cancer (MSI-H, MSI-I and MSS)
“Microsatellite instability is defined as indel (insertion or deletion) mutations within the repetitive regions (microsatellite) in cancer cells by the inactivation of DNA mismatch repair proteins.“ Do you know? Introns …
Role of Microsatellite Instability (MSI) in cancer (MSI-H, MSI-I and MSS) Read More »
