tracrRNA short for Trans-active crRNA binds with the crRNA and guides the CAS protein for effective DNA cleaving. “CRISPR-CAS9 system is an integral part of the bacterial immune system and …
TracrRNA (Trans Activating crRNA)- a Nobel Prize-Winning Finding in CRISPR-Gene Editing Read More »
“PAM stands for Protospacer Adjacent Motif, which functions as two-factor authentication for Cas to cleave the foreign invading DNA only.” The CRISPR system has been an integral part of bacterial …
Importance of PAM Sequence (Protospacer Adjacent Motif) in CRISPR System Read More »
“CRISPR naturally has spacer sequences in-between and works in sync with the CAS protein as an adaptive immune system in bacterial and archaea.” The story of CRISPR and its fascinating …
How does the CRISPR-Mediated Adaptive Immune System Work in Bacteria? Read More »
CRISPRi or CRISPR activation is a process to upregulate gene expression or activate genes by effector activators, dcas9 and sgRNA. In 2021, CRISPR have deep penetration in diverse scientific fields. …
“PCR amplification is an enzyme-dependent process to copy a DNA sequence, DNA fragment, genomic DNA, plasmid DNA or any nucleic acid.“ In other words, we can say, Producing multiple copies …
10 Strategies to Achieve Excellent PCR Amplification Read More »
CRISPRi, short for CRISPR interference, is designed to repress gene expression using the customized sgRNA and dCAS. CRISPR- CAS9 is simply amazing! Why? Because it can edit a gene at …
