“PCR amplification is an enzyme-dependent process to copy a DNA sequence, DNA fragment, genomic DNA, plasmid DNA or any nucleic acid.“ In other words, we can say, Producing multiple copies …
10 Strategies to Achieve Excellent PCR Amplification Read More »
“DNA denaturation is a process to break the double-stranded DNA into single-stranded by either physical or chemical agents is known as DNA denaturation.” For the last couple of weeks, we …
Process to Denature DNA- Definition, Importance, Techniques and Applications Read More »
qPCR acronym as quantitative PCR is a technique to measure the amount of DNA present in a sample. It can study gene expression by utilizing either the TaqMan probe or …
Easiest qPCR Protocol using TaqMan Probe and SYBR Green Dye Read More »
An exclusive native PCR modification known as Asymmetric PCR has been applied to amplify only a single strand during the PCR. PCR is a thermocycler, amplifies DNA. Meaning, it helps …
Asymmetric PCR: Principle, Process, Protocol, Applications, Advantages and Limitations Read More »
“The percent of Guanine and Cytosine, nitrogenous bases present in a DNA, RNA, nucleic acid sequence or primer is known as GC content. The unit to calculate the GC content …
Positive, Negative and Internal controls are common PCR control types employed as a reference to evaluate results. If you know PCR well, you probably understand why we use the controls! …
Type of PCR Controls- Negative, Positive and Internal Controls Read More »
