What is ‘Sequencing Read’ in NGS?
“A sequencing read is referred to as nucleotides of a single DNA fragment from a library. Sequencing reads, read lengths, numbers, read alignments and read scores are important terminologies, technically. …
DNA sequencing
What is Electropherogram? How to Read it?
“A computational/graphical representation of nucleic acid separation generated during high throughput electrophoresis is referred to as an electropherogram.” This article mainly focuses on what is an electropherogram of DNA or …
Differences between Genotyping vs Sequencing
Genotyping is a process of investigating a variant(s) present in an individual while sequencing is a process of investigating the whole sequence, gene or genome. Genotyping and sequencing are separate …
The Concept of ChIP-Seq (ChIP-Sequencing) Explained
“Sequence information of DNA linked with associated proteins like histones can be obtained by performing high throughput DNA sequencing, such technique is known as ChIP-Seq.” Highlights: ChIP-seq was developed by …
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What is the Role of ddNTPs?
The dideoxynucleotides are known as the chain terminator nucleotides that abort the nucleotide chain due to lack of 3’ OH group. Within the past 20 to 25 years the DNA …
DNA fragmentation- Techniques, Importance and Applications
“DNA fragmentation is a technique of artificial breaking of DNA in order to use it in various downstream genetics and well as genomics applications.” The first DNA fragmentation was reported …
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