5 Interpretation Manifests RNA Degradation
“RNA degradation is a common molecular lab problem caused by the fragile nature of RNA and the presence of the RNase enzyme predominantly in nature.” Ribonucleic acid-RNA is a one …
What is RNA Integrity Number (RIN)? and Why It Is Important?
“RIN is an RNA Integrity Number showing the value of how intact the RNA is. RIN score >8.0 is ideally recommended for high throughput downstream processing.” Unlike DNA, RNA is …
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6 Optimizations to Improve the PCR Genotyping
“PCR genotyping is a technique to identify pair of alleles present in a sample thereby the condition- homozygous dominant, homozygous recessive or heterozygous.” Thalassemia and sickle cell anemia-like conditions are …
4 Important Functions of Nuclease-Free Water
“Nuclease-free water is an ultrapure DNase and RNase-free water used in nucleic acid extraction and PCR reaction preparation.” Nucleic acid extraction/ separation is a process to collect or isolate DNA …
Tissue Homogenization Techniques for RNA Extraction
“Tissue homogenization is a process of preparing a homogenous emulsion or suspension of cells through chemical, physical, mechanical or other ways.” RNA extraction is a ‘bone-crushing’ process and a headache …
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How to Isolate mRNA from Total Cellular RNA?
“Only 5% of total cellular RNA content is mRNA and is isolated by removing all other non-coding RNAs from the sample. The unique 3’-OH end of mRNA is utilized to …