A crucial step in genomic DNA extraction in any DNA extraction protocol is to lyse the cell. Breaking the cell wall or nuclear membrane with the help of different types of chemical, physical and enzymatic method is called as lysis or cell lysis. In this article, we are discussing different types of cell lysis
Tris and EDTA are two major components which are used throughout the DNA extraction protocol. Tris and EDTA are applicable in lysis buffer preparation, elution buffer preparation and washing buffer preparation. The major role of TE buffer in DNA extraction is to dissolve DNA into liquid form. However, it is also used as lysis buffer.
Different types of DNA extraction methods are available for different cell types. For example, the DNA extraction method for plant DNA is different from that of the blood. In this article, we are going to discuss different types of DNA extraction methods which are mostly used in genomic labs. We will focus on the principle