Tag: Tris

What are the different components used in the PCR reaction buffer?

The PCR enhancer increase the yield and the accuracy of the PCR result. The PCR reaction buffer enriched with different components such as Tris, EDTA, MgCl2, KCl, Formamide, DMSO, TritonX100, Nonidet P40, twin20, 7-deaza-2′-deoxyguanosine 5′-triphosphate and bovine serum albumin. These are the common PCR enhancers used into the PCR reaction.    “The PCR reaction buffer is

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How to prepare lysis buffer for different types of DNA extraction methods?

A crucial step in genomic DNA extraction in any DNA extraction protocol is to lyse the cell. Breaking the cell wall or nuclear membrane with the help of different types of chemical, physical and enzymatic method is called as lysis or cell lysis.   In this article, we are discussing different types of cell lysis

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Importance of Tris-EDTA (TE) buffer in DNA extraction

Tris and EDTA are two major components which are used throughout the DNA extraction protocol. Tris and EDTA are applicable in lysis buffer preparation, elution buffer preparation and washing buffer preparation. The major role of TE buffer in DNA extraction is to dissolve DNA into liquid form. However, it is also used as lysis buffer.

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