“Quantitative PCR is a technique for nucleic acid quantification. TaqMan Probe and SYBR Green Dye are two common techniques used for qPCR.” In our previous comprehensive article on real-time PCR, …
TaqMan Probe vs SYBR green Dye-based qPCR- How to select the one? Read More »
“The Ct value is a value used to quantify the absolute amount of nucleic acid present in a sample. It is the number of cycles from where fluorescence increases significantly.” …
Explaining the Ct value (Threshold Cycle) of qPCR Read More »
“Real-time PCR is a technique used for the quantification of nucleic acid present in the sample using either fluorescence dye or probe-based technique.” PCR is capable enough to amplify the …
Real-time PCR: Principle, Procedure, Advantages, Limitations and Applications Read More »
