Explaining the Ct value (Threshold Cycle) of qPCR
“The Ct value is a value used to quantify the absolute amount of nucleic acid present in a sample. It is the number of cycles from where fluorescence increases significantly.” …
Continue readingReal-time PCR: Principle, Procedure, Advantages, Limitations and Applications
“A technique used to quantify the nucleic acid (DNA/RNA) present in a sample, during the PCR reaction is known as a real-time PCR or quantitative (q)PCR” In other words, we…
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