The DNA ladder is a standard sized fragment of DNA used to determine the molecular weight of the PCR amplicons. The commercially available DNA ladders come under 50bp, 100bp, 1000bp…
Continue readingIn the present article, we will analyze and interpret agarose gel electrophoresis results of restriction digestion, circular DNA, linear DNA, supercoiled DNA and multiplex PCR. After the great success of…
Continue readingAgarose gel electrophoresis is an important technique in molecular genetics since long. The DNA bands can only be visualised using the agarose gel electrophoresis. In the genomic research, analysing and…
Continue readingAgarose gel electrophoresis: Equipment, Principle, Protocol and Applications: “Electrophoresis is a common genetic lab technique used to separate charged particles such as DNA based on the size of the particle.”…
Continue readingA buffer is an important ingredient in any biological reaction. “A biological buffer solution is a combination of a weak acid and conjugated base or vice verse which helps in…
Continue readingDNA gel loading dye composition, preparation and function. Loading dye is an important component in agarose gel electrophoresis. The loading dye contains bromophenol blue, Ficoll 400 and water majorly while…
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