Month: August 2018

DNA gel loading dye

DNA gel loading dye composition, preparation and function. Loading dye is an important component in agarose gel electrophoresis. The loading dye contains bromophenol blue, Ficoll 400 and water majorly while Xylene cyanol, Tris and EDTA are optional. Bromophenol blue is one of the most popular indicators of DNA in agarose gel electrophoresis. Bromophenol blue is

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Role of EtBr in molecular genetics and cytogenetics

Role of Ethidium bromide in agarose gel electrophoresis and Cytogenetics The role of EtBr in agarose electrophoresis is to intercalate with DNA and helps in the identification of DNA fragments. Yet, it plays a significant role in cytogenetics as well. In this article, We will discuss the role of EtBr in molecular genetics and cytogenetics. Ethidium

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DNA packaging in eukaryotes

Molecular organization of eukaryotic chromosome “The DNA wrapped around histone octamer is called as a nucleosome” In the previous article “ story of chromosome” we had covered the history and structure of the chromosome in brief. The chromosomes are the basic unit of hereditary. DNA is arranged on the chromosome and made up of nitrogenous

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