Month: January 2019

DNA sequencing

Determining the order of the nucleotides present in the DNA is called as a DNA sequencing. Our DNA is made up of the 4 nitrogenous bases adenine, thymine, cytosine and guanine. A long chain of the bases which codes for a particular protein is called as a gene.   For understanding the structure and function

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10 tips on how to do PCR

  Why we always give importance to the PCR? Because it is a key step in genomic research techniques starting from RFLP to the NGS. This is the reason why doing PCR is very important. PCR is one of the routine technique in the molecular genetic labs. A million copies of DNA can be generated

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What are the different components used in the PCR reaction buffer?

The PCR enhancer increase the yield and the accuracy of the PCR result. The PCR reaction buffer enriched with different components such as Tris, EDTA, MgCl2, KCl, Formamide, DMSO, TritonX100, Nonidet P40, twin20, 7-deaza-2′-deoxyguanosine 5′-triphosphate and bovine serum albumin. These are the common PCR enhancers used into the PCR reaction.    “The PCR reaction buffer is

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