Category: Gel electrophoresis

A complete guide for analysing and interpreting gel electrophoresis results

Agarose gel electrophoresis is an important technique in molecular genetics since long. The DNA bands can only be visualised using the agarose gel electrophoresis. In the genomic research, analysing and interpreting the agarose gel electrophoresis results are very crucial. A lot of expertise and experience are required for Interpreting gel electrophoresis results. In this article,

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Agarose gel electrophoresis

Agarose gel electrophoresis: equipment, principle, protocol and application. Agarose electrophoresis is an important technique in genomic research. It separates biological molecules based on their size and charge. It is used for the separation of DNA, RNA, protein and even other biological molecules. In the early 19th century, Johann Wilhelm Hittorf et al., stated that “Under

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Agarose gel electrophoresis buffer

  A buffer is an important ingredient in any biological reaction. “A biological buffer solution is a combination of weak acid and conjugated base or vice verse which helps in maintaining constant pH of the solution”. Tris-borate EDTA and Tris-acetate EDTA are two most common types of buffer solutions used in agarose gel electrophoresis of

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