Category: Gel electrophoresis

Agarose gel electrophoresis

Team Genetic Education 15/09/2018 No Comments All articles, Explore, Gel electrophoresis , , , ,

Agarose gel electrophoresis: equipment, principle, protocol and application. Agarose electrophoresis is an important technique in genomic research. It separates biological molecules based on their size and charge. It is used for the separation of DNA, RNA, protein and even other biological molecules. In the early 19th century, Johann Wilhelm Hittorf et al., stated that “Under […]

Agarose gel electrophoresis buffer

Team Genetic Education 03/09/2018 No Comments All articles, Gel electrophoresis , , , , , , ,

  A buffer is an important ingredient in any biological reaction. “A biological buffer solution is a combination of weak acid and conjugated base or vice verse which helps in maintaining constant pH of the solution”. Tris-borate EDTA and Tris-acetate EDTA are two most common types of buffer solutions used in agarose gel electrophoresis of […]

DNA gel loading dye

Team Genetic Education 30/08/2018 No Comments All articles, Educational articles, Gel electrophoresis , , , , , , , ,

DNA gel loading dye composition, preparation and function. Loading dye is an important component in agarose gel electrophoresis. The loading dye contains bromophenol blue, Ficoll 400 and water majorly while Xylene cyanol, Tris and EDTA are optional. Bromophenol blue is one of the most popular indicators of DNA in agarose gel electrophoresis. Bromophenol blue is […]

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