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Category: PCR technology

Function of taq DNA polymerase in PCR

The function of Taq DNA polymerase in PCR reaction is to amplify the DNA for the production of multiple copies of DNA. Taq DNA polymerase is a thermostable DNA polymerase which can also work at a higher temperature. In this article, we will discuss about the DNA polymerase and its function, Taq DNA polymerase, the function

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PCR primer design guidelines

PCR primer design guideline: PCR primers are similar as like primer involved in DNA replication in vivo but the major difference between both types of primers are, PCR primers are DNA primers while the in vivo replication primers are RNA primers. In this article, we are discussing the PCR primer and their properties along with

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Role of MgCl2 in PCR reaction

The Role of MgCl2 in PCR reaction is to enhance the DNA amplification by boosting the activity of Taq DNA polymerase. Polymerase chain reaction is one of the important experimental procedure in downstream genomic research. It generates millions of amplicon for the gene of our interest. The process is as similar to replication but it

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