“Nested PCR uses two sets of primers and increases the specificity of the PCR reaction manyfold. And thus reduces the chances of false-positive results.” If someone asks you, what is […]
What is Nested PCR?- Concept, Primers, Protocol, Advantages and Limitations Read More »
“Hot start PCR is a modification of conventional PCR in which limiting one of the reagents until the heating step reduces the chances of non-specific amplification.” Non-specific binding or non-specific
What is a Hot-Start PCR? Read More »
“Multiplex PCR amplifies multiple DNA template regions, simultaneously using different sets of primers in a single PCR reaction.” PCR technology has the best utility in research as well as in
Multiplex PCR- Principle, Process, Protocol, Advantages, Limitations and Applications Read More »
“A method used to detect a single base change or SNP using sequence-specific primers is referred to as ARMS or Allele-specific PCR.” The PCR technique can detect mutations like deletion,
Immuno PCR/IPCR/qIPCR is a “combination of the efficiency of RT-PCR and specificity of the ELISA method”. As the name suggests the method is a combination of immunology and genetics. The
What is Immuno-PCR or IPCR? Read More »
“Plant DNA extraction needs a CTAB DNA extraction buffer that contains ingredients that can effectively remove polysaccharides and polyphenols. Let’s see how it works and how to optimize the CTAB
Plant DNA Extraction using CTAB Buffer Read More »
