Sanger sequencing is a gold-standard DNA sequencing technique that has been employed for years in genetic research and diagnosis. This category explains the concept, principle, chemistry, automation, optimizations, troubleshooting and results and analysis in detail.
Explore all the articles on Sanger sequencing and master this essential molecular genetic skill.
“The quality of Sanger sequencing results highly relies on the quality of the template used during sequencing. Learn how to prepare a sample for Sanger sequencing in this guide.” One […]
Template preparation for Sanger sequencing- A step-by-step Guide Read More »
“The Sanger sequencing technique is a gold-standard method for DNA sequencing and is widely used routinely in various fields.” Sequencing techniques allow scientists to determine or ready nucleotides from any
Advances in Sanger Sequencing- Manual vs Automated Sanger Sequencing Read More »
“A Sanger sequencing gel carries many DNA bands for each reaction and is thus complicated to study. In this article, Learn how to interpret a Sanger Sequencing gel with thumb
A Step-by-Step Process on How to Read Sanger Sequencing Gel? Read More »
“Sanger sequencing is a genetic technique to determine the order of nucleotides while PCR generates copies of the DNA fragment, present in the sample. Let’s see some of the significant
Sanger Sequencing vs PCR: Common and Technical Differences Read More »
“Parameters that influence the Sanger sequencing results are good quality extract, amplicon purification, primer designing and managing leftover ddNPTs. These parameters can improve the Sanger Sequencing results, certainly.” Let’s findout
How to improve Sanger Sequencing Results?- 5 Technical Tips from Experts Read More »
“ddNTPs are known as chain termination nucleotides. In this article, learn about what ddNTPs are, why we are using them, and the difference between ddNTP and dNTP.” PCR and DNA
ddNTP in Sanger Sequencing- What Is It and Why We Use It? Read More »
