Tag: PCR

PCR primer design guidelines

Team Genetic Education 19/08/2018 No Comments All articles, Explore, PCR technology , , , ,

PCR primer design guideline: PCR primers are similar as like primer involved in DNA replication in vivo but the major difference between both types of primers are, PCR primers are DNA primers while the in vivo replication primers are RNA primers. In this article, we are discussing the PCR primer and their properties along with […]

Role of MgCl2 in PCR reaction

Team Genetic Education 06/08/2018 1 Comment All articles, PCR technology , , , , ,

The Role of MgCl2 in PCR reaction is to enhance the DNA amplification by boosting the activity of Taq DNA polymerase. Polymerase chain reaction is one of the important experimental procedure in downstream genomic research. It generates millions of amplicon for the gene of our interest. The process is as similar to replication but it […]

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