Usually, 25 to 35 cycles a typical PCR reaction needs to get a good amount of amplification, containing denaturation, annealing and extension simultaneously at 95ºC, 50 to 68ºC and 72ºC. […]
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“Genetics is a branch of science that involve the study of genes, DNA, chromosomes and related inherited disorders.” It is a science of studying the inheritance of genes and genotypes.
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“PCR is one of the most versatile, robust and widely utilized techniques used in various fields of science starting from genomic science to microbiology and food industry. It is used
50 Powerful Applications of PCR Read More »
“In a gradient PCR, we can create temperature variation for the annealing step, set different annealing temperatures and test different parameters at different temperatures.” PCR is an amazing discovery in
What is Gradient PCR and How to Use it? Read More »
“If you know the process of sequencing, amplification by PCR is a dedicated step. Though PCR has its own importance, it is utilized in sequencing for various purposes. Let’s find
Why is PCR used in the Process of DNA Sequencing? Read More »
“The PCR test is performed to get millions of copies of template DNA for performing different genetic applications using the heat dependent rapid amplification method.” Why we always give importance
PCR Test: 10 tips on how to do it Read More »
