What should be the key requirement to perform any molecular genetic experiment in which DNA is involved? Your answer will be ‘DNA’, Right? Let me tell you that you are […]
How to Improve Your PCR or qPCR Results? [Skills assessment] Read More »
Do you know a typical NGS machine can generate around 1.8 to 6TB of sequencing data in a single run? PCR duplication is a serious problem in so many crucial
Emulsion PCR: Principle, Process, Advantages, Applications and Limitations Read More »
“Primer dimers are unintended short amplification products formed in the PCR by unhealthy PCR practices. This article covers common causes for primer dimer formation and troubleshooting.” In the previous article
PCR Troubleshooting 103: How to Address Primer-Dimers Read More »
“Numerous genetic testing techniques and assays are now available, however, selecting one for your experiment is a crucial decision. This article provides a comprehensive guide for various genetic methods and
How to Select The Most Effective Genetic Assay? Read More »
“Allelic dropout in the PCR is a condition in which one or both the alleles in the heterozygous condition fail to amplify and cause a fault in results. In this
PCR Troubleshooting 102: How to Address The Allelic Dropout Read More »
“Non-specific amplification is one of the most common problems in any PCR reaction. In this guide, I will explain the reasons and possible (technical) solutions to overcome non-specific amplification.” Imagine,
PCR Troubleshooting 101: How to Address Non-Specific Amplification? Read More »
