What should be the key requirement to perform any molecular genetic experiment in which DNA is involved? Your answer will be ‘DNA’, Right? Let me tell you that you are …
How to Improve Your PCR or qPCR Results? [Skills assessment] Read More »
Do you know a typical NGS machine can generate around 1.8 to 6TB of sequencing data in a single run? PCR duplication is a serious problem in so many crucial …
Emulsion PCR: Principle, Process, Advantages, Applications and Limitations Read More »
“Primer dimers are unintended short amplification products formed in the PCR by unhealthy PCR practices. This article covers common causes for primer dimer formation and troubleshooting.” In the previous article …
PCR Troubleshooting 103: How to Address Primer-Dimers Read More »
“Numerous genetic testing techniques and assays are now available, however, selecting one for your experiment is a crucial decision. This article provides a comprehensive guide for various genetic methods and …
“Allelic dropout in the PCR is a condition in which one or both the alleles in the heterozygous condition fail to amplify and cause a fault in results. In this …
PCR Troubleshooting 102: How to Address The Allelic Dropout Read More »
“Non-specific amplification is one of the most common problems in any PCR reaction. In this guide, I will explain the reasons and possible (technical) solutions to overcome non-specific amplification.” Imagine, …
PCR Troubleshooting 101: How to Address Non-Specific Amplification? Read More »
