“Quantitative PCR is a technique for nucleic acid quantification. TaqMan Probe and SYBR Green Dye are two common techniques used for qPCR.” In our previous comprehensive article on real-time PCR, …
TaqMan Probe vs SYBR green Dye-based qPCR- How to select the one? Read More »
What should be the key requirement to perform any molecular genetic experiment in which DNA is involved? Your answer will be ‘DNA’, Right? Let me tell you that you are …
How to Improve Your PCR or qPCR Results? [Skills assessment] Read More »
Do you know a typical NGS machine can generate around 1.8 to 6TB of sequencing data in a single run? PCR duplication is a serious problem in so many crucial …
Emulsion PCR: Principle, Process, Advantages, Applications and Limitations Read More »
“False positive and false negative results in PCR are two critical problems. In this article, learn about these two common PCR errors, how it occurs, and how to overcome them.” …
PCR Troubleshooting 104: Understanding False Positive and False Negative PCR Results Read More »
“Primer dimers are unintended short amplification products formed in the PCR by unhealthy PCR practices. This article covers common causes for primer dimer formation and troubleshooting.” In the previous article …
PCR Troubleshooting 103: How to Address Primer-Dimers Read More »
“Detergents play a crucial role in any DNA extraction scheme and thus have pivotal importance. This article focuses on the role and types of detergents used in DNA extraction.” Do …
What is the role of Detergents in DNA extraction? Read More »
