What is Immuno-PCR or IPCR?
Immuno PCR/IPCR/qIPCR is a “combination of the efficiency of RT-PCR and specificity of the ELISA method”. As the name suggests the method is a combination of immunology and genetics. The […]
PCR Technology
PCR stands for Polymerase chain reaction. It is a laboratory genetic technique used to amplify DNA and generate copies for downstream analysis. Learn PCR, its principle, steps, advanced protocols, troubleshooting, types and other related information in this category.
Explore all articles on PCR technology and master this essential molecular skill.
PCR Test: 10 tips on how to do it
“The PCR test is performed to get millions of copies of template DNA for performing different genetic applications using the heat dependent rapid amplification method.” Why we always give importance
What are the Different Components used in the PCR Reaction Buffer?
“The PCR reaction buffer is enriched with the PCR enhancers that increase the overall reaction performance, some of them are Tris, EDTA, MgCl2, KCl, Formamide, DMSO, TritonX100, Nonidet P40, twin20,
What are the Different Components used in the PCR Reaction Buffer? Read More »
Polymerase Chain Reaction (PCR)- Definition, Principle, Steps, Procedure, Protocol, Applications and Types
“The Polymerase chain reaction is an in vitro DNA synthesis method in which DNA is amplified using the Taq DNA polymerase enzyme.” Related article: 10 Strategies to Achieve Excellent PCR
The Function of dNTPs in PCR Reaction
dNTP stands for deoxyribose nucleotide triphosphate employed in PCR to expand the growing DNA strand. dATP, dTTP, dGTP and dTTP are four common dNTPs used in PCR. The function of
Role of DMSO in PCR
DMSO is an important PCR enhancer and an ingredient of PCR buffer. It increases the yield and specificity of the reaction. Let’s find out what its function is and, why