Real-time PCR, often known as quantitative PCR, is a revolutionary technique to monitor DNA amplification in real-time. It is extensively used in diagnostics, epigenetic studies, microbial detection and mutation studies. Learn the fundamentals, theory, techniques, chemistries, problems, troubleshooting and applications of real-time PCR in this category.
Explore all articles on real-time PCR and master this essential genetic skill.
“Quantitative PCR is a technique for nucleic acid quantification. TaqMan Probe and SYBR Green Dye are two common techniques used for qPCR.” In our previous comprehensive article on real-time PCR, […]
TaqMan Probe vs SYBR green Dye-based qPCR- How to select the one? Read More »
qPCR acronym as quantitative PCR is a technique to measure the amount of DNA present in a sample. It can study gene expression by utilizing either the TaqMan probe or
Easiest qPCR Protocol using TaqMan Probe and SYBR Green Dye Read More »
“A Higher Ct value indicates a lower viral load whilst the lower Ct value indicates a higher amount of viral load in the sample.” I went twice for RT-PCR in
What is the Ct value of SARS-CoV-2 (COVID-19) RT-PCR? Read More »
“The Ct value is a value used to quantify the absolute amount of nucleic acid present in a sample. It is the number of cycles from where fluorescence increases significantly.”
Explaining the Ct value (Threshold Cycle) of qPCR Read More »
“PCR- the polymerase chain reaction is one of the most versatile and state of the art techniques used to amplify and quantify DNA.” The PCR machine known as a thermocycler
Differences between PCR vs qPCR Read More »
DNA probes are single-stranded and labeled oligonucleotide sequences used in FISH, PCR and DNA sequencing. Learn about DNA probes, types, examples and various types of labeling techniques. Also, explore applications
DNA Probes: Labeling, Types, Applications and Limitations Read More »
