“Primer dimers are short amplified products formed by the binding of primers during PCR amplification. Let’s learn what primer dimers are in PCR, qPCR, gel electrophoresis and DNA sequencing.” PCR […]
What are Primer Dimers? – A Beginner’s Guide Read More »
“In a gradient PCR, we can create temperature variation for the annealing step, set different annealing temperatures and test different parameters at different temperatures.” PCR is an amazing discovery in
What is Gradient PCR and How to Use it? Read More »
“Countless commercially available DNA polymerases make the choice difficult. Here is the guide on how to select a DNA Polymerase for your genetic experiment.” The function of a DNA polymerase
A Guide to Choose DNA Polymerase for Your Experiment Read More »
RNA, Ribonucleic acid is a type of nucleic acid involved in protein synthesis. In the present article, we are briefly discussing each type of RNA with its function and general
RNA: Structure, Types and Function Read More »
“The site-directed mutagenesis is an artificial (in vitro) technique for introducing mutation or alteration into the target (known) DNA sequence.” In simple words, we can say that by using artificial
Site-Directed Mutagenesis: Methods and Applications Read More »
“RT-PCR can effectively quantify copies of a gene present in the sample. Thus, it’s widely used in gene expression studies, cancer research and viral load estimation.” PCR (Polymerase Chain Reaction)
