PCR stands for Polymerase chain reaction. It is a laboratory genetic technique used to amplify DNA and generate copies for downstream analysis. Learn PCR, its principle, steps, advanced protocols, troubleshooting, types and other related information in this category.
Explore all articles on PCR technology and master this essential molecular skill.
“The PCR reaction buffer is enriched with the PCR enhancers that increase the overall reaction performance, some of them are Tris, EDTA, MgCl2, KCl, Formamide, DMSO, TritonX100, Nonidet P40, twin20, […]
What are the Different Components used in the PCR Reaction Buffer? Read More »
“The Polymerase chain reaction is an in vitro DNA synthesis method in which DNA is amplified using the Taq DNA polymerase enzyme.” Related article: 10 Strategies to Achieve Excellent PCR
dNTP stands for deoxyribose nucleotide triphosphate employed in PCR to expand the growing DNA strand. dATP, dTTP, dGTP and dTTP are four common dNTPs used in PCR. The function of
The Function of dNTPs in PCR Reaction Read More »
DMSO is an important PCR enhancer and an ingredient of PCR buffer. It increases the yield and specificity of the reaction. Let’s find out what its function is and, why
Role of DMSO in PCR Read More »
“The function of Taq DNA polymerase in PCR is to amplify or synthesize DNA or gene of interest for various downstream applications. It’s a type of thermostable DNA polymerase, work
Function of Taq DNA Polymerase in PCR Read More »
PCR primers are short single-stranded, specific/ complementary DNA sequences, providing the free 3’ OH end for synthesis during the PCR reaction. Polymerase Chain Reaction- PCR, is an in vitro DNA
How to Design PCR Primers Read More »
