PCR stands for Polymerase chain reaction. It is a laboratory genetic technique used to amplify DNA and generate copies for downstream analysis. Learn PCR, its principle, steps, advanced protocols, troubleshooting, types and other related information in this category.
Explore all articles on PCR technology and master this essential molecular skill.
“Discover the role of an RNA primer and a DNA primer in two different DNA synthesis processes. Learn about each type of primer including their significance and applications, in this
Similarities and Differences between DNA Primers vs RNA Primers Read More »
“The DNA ladder is a standard-sized molecular marker or fragments of DNA applied to determine the size of PCR amplicons. 50bp, 100bp, 1000bp and 3000bp are several commercially available and
What is a DNA ladder? and How To Prepare It? (A Complete Protocol) Read More »
“A hairpin-shaped, single-stranded, sequence-specific real-time PCR probe is known as Molecular Beacon having a higher sensitivity and specificity.” The present article is more technical, including in-depth technical things related to
Structure and Function of Molecular Beacon Probes Read More »
“A typical PCR reaction contains a required concentration of dNTPs, Taq DNA polymerase, PCR primers, PCR buffer, template DNA and water. Each PCR reaction varies in terms of the concentration
How to Prepare an Excellent PCR Reaction? A step-by-step Guide Read More »
“Primer dimers are short amplified products formed by the binding of primers during PCR amplification. Let’s learn what primer dimers are in PCR, qPCR, gel electrophoresis and DNA sequencing.” PCR
“In a gradient PCR, we can create temperature variation for the annealing step, set different annealing temperatures and test different parameters at different temperatures.” PCR is an amazing discovery in
