What is TB-PCR?
“The TB PCR method is the gold standard method in the detection of Tuberculosis infection.” In this method, the insertion sequence of the Tuberculosis is amplified using the polymerase chain […]
PCR Technology
PCR stands for Polymerase chain reaction. It is a laboratory genetic technique used to amplify DNA and generate copies for downstream analysis. Learn PCR, its principle, steps, advanced protocols, troubleshooting, types and other related information in this category.
Explore all articles on PCR technology and master this essential molecular skill.
What is in situ PCR?
“The in situ PCR or slide PCR is a method used to amplify the DNA present in the paraffin-embedded tissue or formalin-fixed tissue on the glass slide.” In the
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What is Nested PCR?- Concept, Primers, Protocol, Advantages and Limitations
“Nested PCR uses two sets of primers and increases the specificity of the PCR reaction manyfold. And thus reduces the chances of false-positive results.” If someone asks you, what is
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What is a Hot-Start PCR?
“Hot start PCR is a modification of conventional PCR in which limiting one of the reagents until the heating step reduces the chances of non-specific amplification.” Non-specific binding or non-specific
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Multiplex PCR- Principle, Process, Protocol, Advantages, Limitations and Applications
“Multiplex PCR amplifies multiple DNA template regions, simultaneously using different sets of primers in a single PCR reaction.” PCR technology has the best utility in research as well as in
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ARMS or Allele-Specific PCR- Principle, Procedure, Protocol, Applications and Limitations
“A method used to detect a single base change or SNP using sequence-specific primers is referred to as ARMS or Allele-specific PCR.” The PCR technique can detect mutations like deletion,