PCR troubleshooting

Troubleshooting allelic dropout in the PCR.

Apr 12 2023

PCR Troubleshooting 102: How to Address The Allelic Dropout

PCR technology / By Dr Tushar Chauhan / 12/04/2023

“Allelic dropout in the PCR is a condition in which one or both the alleles in the heterozygous condition fail to amplify and cause a fault in results. In this …

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PCR troubleshooting for non-specific amplification.

Apr 5 2023

PCR Troubleshooting 101: How to Address Non-Specific Amplification?

PCR technology / By Dr Tushar Chauhan / 05/04/2023

“Non-specific amplification is one of the most common problems in any PCR reaction. In this guide, I will explain the reasons and possible (technical) solutions to overcome non-specific amplification.” Imagine, …

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