“PAM stands for Protospacer Adjacent Motif, which functions as two-factor authentication for Cas to cleave the foreign invading DNA only.” The CRISPR system has been an integral part of bacterial […]
Importance of PAM Sequence (Protospacer Adjacent Motif) in CRISPR System Read More »
“CRISPR naturally has spacer sequences in-between and works in sync with the CAS protein as an adaptive immune system in bacterial and archaea.” The story of CRISPR and its fascinating
How does the CRISPR-Mediated Adaptive Immune System Work in Bacteria? Read More »
CRISPRi or CRISPR activation is a process to upregulate gene expression or activate genes by effector activators, dcas9 and sgRNA. In 2021, CRISPR have deep penetration in diverse scientific fields.
What is CIRSPRa or CRISPR activation? Read More »
“PCR amplification is an enzyme-dependent process to copy a DNA sequence, DNA fragment, genomic DNA, plasmid DNA or any nucleic acid.“ In other words, we can say, Producing multiple copies
10 Strategies to Achieve Excellent PCR Amplification Read More »
CRISPRi, short for CRISPR interference, is designed to repress gene expression using the customized sgRNA and dCAS. CRISPR- CAS9 is simply amazing! Why? Because it can edit a gene at
What is CRISPRi or CRISPR interference? Read More »
“dCAS9 is a deactivated or dead Cas protein that lacks nucleophilic activity or simply is a deactivated nuclease CAS9.” Read more: CRISPR-Cpf1(Cas12a) Gene-Editing System- Explained. You may wonder, Without nucleophilic
dCAS9 (Dead CAS system): Concept, Functions and Applications Read More »
