CRISPR-CAS9 in the Treatment of Ataxia Type 2
“The Year 2020, was the grand time for Ataxia treatment based on the CRISPR-based technology. Three consecutive studies on Ataxia type 1, type 2 and type 3 proving the CRISPR […]
TracrRNA (Trans Activating crRNA)- a Nobel Prize-Winning Finding in CRISPR-Gene Editing
tracrRNA short for Trans-active crRNA binds with the crRNA and guides the CAS protein for effective DNA cleaving. “CRISPR-CAS9 system is an integral part of the bacterial immune system and
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Importance of PAM Sequence (Protospacer Adjacent Motif) in CRISPR System
“PAM stands for Protospacer Adjacent Motif, which functions as two-factor authentication for Cas to cleave the foreign invading DNA only.” The CRISPR system has been an integral part of bacterial
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How does the CRISPR-Mediated Adaptive Immune System Work in Bacteria?
“CRISPR naturally has spacer sequences in-between and works in sync with the CAS protein as an adaptive immune system in bacterial and archaea.” The story of CRISPR and its fascinating
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What is CIRSPRa or CRISPR activation?
CRISPRi or CRISPR activation is a process to upregulate gene expression or activate genes by effector activators, dcas9 and sgRNA. In 2021, CRISPR have deep penetration in diverse scientific fields.
10 Strategies to Achieve Excellent PCR Amplification
“PCR amplification is an enzyme-dependent process to copy a DNA sequence, DNA fragment, genomic DNA, plasmid DNA or any nucleic acid.“ In other words, we can say, Producing multiple copies
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