What are Primer Dimers? – A Beginner’s Guide
“Primer dimers are short amplified products formed by the binding of primers during PCR amplification. Let’s learn what primer dimers are in PCR, qPCR, gel electrophoresis and DNA sequencing.” PCR […]
What is Gradient PCR and How to Use it?
“In a gradient PCR, we can create temperature variation for the annealing step, set different annealing temperatures and test different parameters at different temperatures.” PCR is an amazing discovery in
A Guide to Choose DNA Polymerase for Your Experiment
“Countless commercially available DNA polymerases make the choice difficult. Here is the guide on how to select a DNA Polymerase for your genetic experiment.” The function of a DNA polymerase
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Site-Directed Mutagenesis: Methods and Applications
“The site-directed mutagenesis is an artificial (in vitro) technique for introducing mutation or alteration into the target (known) DNA sequence.” In simple words, we can say that by using artificial
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Reverse Transcription PCR: Principle, Procedure, Protocol, Advantages, Limitations, Applications
“RT-PCR can effectively quantify copies of a gene present in the sample. Thus, it’s widely used in gene expression studies, cancer research and viral load estimation.” PCR (Polymerase Chain Reaction)
Real-time PCR: Principle, Procedure, Advantages, Limitations and Applications
“Real-time PCR is a technique used for the quantification of nucleic acid present in the sample using either fluorescence dye or probe-based technique.” PCR is capable enough to amplify the
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