What is in situ PCR?
“The in situ PCR or slide PCR is a method used to amplify the DNA present in the paraffin-embedded tissue or formalin-fixed tissue on the glass slide.” In the […]
What is Nested PCR?- Concept, Primers, Protocol, Advantages and Limitations
“Nested PCR uses two sets of primers and increases the specificity of the PCR reaction manyfold. And thus reduces the chances of false-positive results.” If someone asks you, what is
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What is a Hot-Start PCR?
“Hot start PCR is a modification of conventional PCR in which limiting one of the reagents until the heating step reduces the chances of non-specific amplification.” Non-specific binding or non-specific
Multiplex PCR- Principle, Process, Protocol, Advantages, Limitations and Applications
“Multiplex PCR amplifies multiple DNA template regions, simultaneously using different sets of primers in a single PCR reaction.” PCR technology has the best utility in research as well as in
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ARMS or Allele-Specific PCR- Principle, Procedure, Protocol, Applications and Limitations
“A method used to detect a single base change or SNP using sequence-specific primers is referred to as ARMS or Allele-specific PCR.” The PCR technique can detect mutations like deletion,
What is Immuno-PCR or IPCR?
Immuno PCR/IPCR/qIPCR is a “combination of the efficiency of RT-PCR and specificity of the ELISA method”. As the name suggests the method is a combination of immunology and genetics. The