Explaining CRISPR-CAS9 System- Step by Step
“CRISPR-CAS9 is a revolutionary system of gene editing or genome editing that uses the guided RNA to cleave the DNA at a specific location in a genome.” Editing a gene …
Genetic Engineering
What is sgRNA? – Definition, Designing and Advantages
“The sgRNA or gRNA is known as single-stranded guided RNA or guided RNA used in the gene-editing technique which makes a complex with the CAS9 protein to cleave the DNA.” …
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Genetically Modified Organisms (GMO)- Definition, Process and Examples
“GMOs- genetically modified organisms are any living entity constructed by altering their genetic composition, without affecting their normal function.” In other words, it can be stated as, “Organisms whose genetic …
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RNA Sequencing- Principle, Steps, Methods And Applications
“RNA sequencing is a high throughput next-generation sequencing method used to analyse gene expression and transcriptomics studies.” Ribonucleic acid is a type of nucleic acid majorly involved in the gene …
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How To Construct Knockout Mice? A Stepwise Guide
“A genetically constructed mice in which one of the functional genes is inactivated or knocked out for studying a particular function is called knockout mice.” In 1989, Mario R Capecchi, …
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What is Gene Editing and CRISPR-CAS9?
“Gene-editing is a technique to manipulate an organism’s genome or gene or DNA sequence which might be insertion or deletion. CRISPR-CAS9 system is one of the popular gene-editing techniques so …
