Explaining CRISPR-CAS9 System- Step by Step
“CRISPR-CAS9 is a revolutionary system of gene editing or genome editing that uses the guided RNA to cleave the DNA at a specific location in a genome.” Why are we…
Continue readingWhat is sgRNA? – Definition, Designing and Advantages
“The sgRNA or gRNA is known as single-stranded guided RNA or guided RNA used in the gene-editing technique which makes a complex with the CAS9 protein to cleave the DNA.” …
Continue readingGenetically Modified Organisms (GMO)- Definition, Process and Examples
“GMOs- genetically modified organisms are any living entity constructed by altering their genetic composition, without affecting their normal function.” In other words, it can be stated as, “Organisms whose genetic…
Continue readingRNA Sequencing- Principle, Steps, Methods And Applications
“RNA sequencing is a high throughput next-generation sequencing method used to analyse gene expression and transcriptomics studies.” Ribonucleic acid is a type of nucleic acid majorly involved in the gene…
Continue readingHow To Construct Knockout Mice? A Stepwise Guide
“A genetically constructed mice in which one of the functional genes is inactivated or knocked out for studying a particular function is called knockout mice.” In 1989, Mario R Capecchi,…
Continue readingWhat is Gene Editing and CRISPR-CAS9?
“Gene-editing is a technique to manipulate an organism’s genome or gene or DNA sequence which might be insertion or deletion. CRISPR-CAS9 system is one of the popular gene-editing techniques so…
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