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What is sgRNA? – Definition, Designing and Advantages 

Dr Tushar Chauhan 03/08/2020 0 Comments

“The sgRNA or gRNA is known as single-stranded guided RNA or guided RNA used in the gene-editing technique which makes a complex with the CAS9 protein to cleave the DNA.” …

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Genetic Education Genetic Engineering

Genetically Modified Organisms (GMO)- Definition, Process and Examples

Dr Tushar Chauhan 20/04/2020 0 Comments

“GMOs- genetically modified organisms are any living entity constructed by altering their genetic composition, without affecting their normal function.” In other words, it can be stated as, “Organisms whose genetic…

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DNA Genetic Education Genetic Engineering RNA

RNA Sequencing- Principle, Steps, Methods And Applications 

Dr Tushar Chauhan 06/01/2020 0 Comments

“RNA sequencing is a high throughput next-generation sequencing method used to analyse gene expression and transcriptomics studies.”  Ribonucleic acid is a type of nucleic acid majorly involved in the gene…

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Genetic Education Genetic Engineering

How To Construct Knockout Mice? A Stepwise Guide 

Dr Tushar Chauhan 04/12/2019 0 Comments

“A genetically constructed mice in which one of the functional genes is inactivated or knocked out for studying a particular function is called knockout mice.”  In 1989, Mario R Capecchi,…

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Gene therapy Genes Genetic Education Genetic Engineering

What is Gene Editing and CRISPR-CAS9?

Dr Tushar Chauhan 29/12/2018 0 Comments

“Gene-editing is a technique to manipulate an organism’s genome or gene or DNA sequence which might be insertion or deletion. CRISPR-CAS9 system is one of the popular gene-editing techniques so…

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