How to Design PCR Primers
PCR primers are short single-stranded, specific/ complementary DNA sequences, providing the free 3’ OH end for synthesis during the PCR reaction. Polymerase Chain Reaction- PCR, is an in vitro DNA […]
Genetics
DNA Fingerprinting- Definition, Steps, Methods and Applications
“A laboratory genetic technique or method to identify individuals using bodily samples such as blood, saliva, or hair is referred to as DNA fingerprinting.” Or we can define DNA fingerprinting
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Different Types of Genetic Inheritance Patterns
“Inheritance or passing of a genetic trait or disease gene from one generation to another is referred to as genetic inheritance.” Depending upon the cell types, the genetic inheritance can
Prokaryotic DNA Replication: Initiation, Elongation and Termination
In 1963, J Cairns reported the process of replication in E.coli bacteria by autoradiography. Replication is a process in which a DNA molecule is copied. From the double-stranded DNA, two daughter
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What is the Function of MgCl2 in PCR?
MgCl2 is an important gradient of PCR buffer, a proven PCR enhancer- MgCl2 has many roles in a PCR that certainly lifts the reaction efficiency by many folds. Let’s find
DNA Topology- What is the Linking Number of DNA?
The linking number is a topological property of DNA. A linking number is a sum of twists and writhes. The number of times one strand of DNA turned around another
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