PCR stands for Polymerase chain reaction. It is a laboratory genetic technique used to amplify DNA and generate copies for downstream analysis. Learn PCR, its principle, steps, advanced protocols, troubleshooting, types and other related information in this category.
Explore all articles on PCR technology and master this essential molecular skill.
“PCR master mix is a pre-mix that includes all the essential PCR ingredients except template DNA and primers. Learn its importance and how to prepare it in your lab.” Polymerase […]
[DIY] How to Prepare Your Own PCR Master Mix Read More »
“Multiplex PCR provides flexibility to multiplex the sample for many markers. Here is a list of 10 examples in which you can use a multiplex PCR approach.” PCR is a
When to Use a Multiplex PCR? Explained by 8 Real Applications Read More »
“Learn the technical process of ordering, storing and using the PCR primer for the PCR reaction.” In the previous article on PCR primers, we learned about the concept of PCR
How to Order, Store and Use PCR Primers? Read More »
“Quantitative PCR is a technique for nucleic acid quantification. TaqMan Probe and SYBR Green Dye are two common techniques used for qPCR.” In our previous comprehensive article on real-time PCR,
TaqMan Probe vs SYBR green Dye-based qPCR- How to select the one? Read More »
What should be the key requirement to perform any molecular genetic experiment in which DNA is involved? Your answer will be ‘DNA’, Right? Let me tell you that you are
How to Improve Your PCR or qPCR Results? [Skills assessment] Read More »
Do you know a typical NGS machine can generate around 1.8 to 6TB of sequencing data in a single run? PCR duplication is a serious problem in so many crucial
Emulsion PCR: Principle, Process, Advantages, Applications and Limitations Read More »
