“By partitioning the sample into smaller volumes, absolute quantification of nucleic acid is performed with high accuracy and precision using digital PCR.” The foremost application of PCR is to either…
Continue reading“PCR is one of the most versatile, robust and widely utilized techniques used in various fields of science starting from genomic science to microbiology and food industry. It is used…
Continue readingPrimers in molecular biology are used as a start point in DNA synthesis, in vitro as well as in vivo. The DNA primer is used in PCR amplification while the…
Continue readingThe DNA ladder is a standard sized fragment of DNA used to determine the molecular weight of the PCR amplicons. The commercially available DNA ladders come under 50bp, 100bp, 1000bp…
Continue readingA molecular beacon is the hairpin-shaped single-stranded structured probe which is highly sequence-specific and sensitive. The Molecular Beacons are used in the real-time PCR assay for the quantification of RNA…
Continue readingPreparing a PCR reaction is one of the important steps in PCR amplification. Taq DNA polymerase, dNTPs, PCR reaction buffer, PCR primer and template DNA are important ingredients of PCR…
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