The DNA ladder is a standard sized fragment of DNA used to determine the molecular weight of the PCR amplicons. The commercially available DNA ladders come under 50bp, 100bp, 1000bp…
Continue readingA molecular beacon is the hairpin-shaped single-stranded structured probe which is highly sequence-specific and sensitive. The Molecular Beacons are used in the real-time PCR assay for the quantification of RNA…
Continue readingPreparing the PCR reaction is one of the important steps in PCR amplification. Taq DNA polymerase, dNTPs, PCR reaction buffer, PCR primer and template DNA are important ingredients of PCR…
Continue readingThe primer dimer is a non-specific product occurred due to the hybridization of several bases of complementary primers during the PCR reaction. The primer dimers are one of the most…
Continue readingIn a single experiment, The best PCR amplification can be achieved by determining the optimum annealing temperature using the gradient PCR. The gradient PCR is not actually a type of…
Continue readingDNA polymerase helps in replicating DNA properly, in PCR, it incorporates dNTPs on the DNA strand. Taq DNA polymerase is the first choice for it. However, the Taq DNA polymerase…
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