“Non-specific amplification is one of the most common problems in any PCR reaction. In this guide, I will explain the reasons and possible (technical) solutions to overcome non-specific amplification.” Imagine, […]
PCR Troubleshooting 101: How to Address Non-Specific Amplification? Read More »
“Sanger sequencing is a genetic technique to determine the order of nucleotides while PCR generates copies of the DNA fragment, present in the sample. Let’s see some of the significant
Sanger Sequencing vs PCR: Common and Technical Differences Read More »
“PCR genotyping is a technique to identify pair of alleles present in a sample thereby the condition- homozygous dominant, homozygous recessive or heterozygous.” Thalassemia and sickle cell anemia-like conditions are
6 Optimizations to Improve the PCR Genotyping Read More »
“PCR amplification is an enzyme-dependent process to copy a DNA sequence, DNA fragment, genomic DNA, plasmid DNA or any nucleic acid.“ In other words, we can say, Producing multiple copies
10 Strategies to Achieve Excellent PCR Amplification Read More »
“DNA denaturation is a process to break the double-stranded DNA into single-stranded by either physical or chemical agents is known as DNA denaturation.” For the last couple of weeks, we
Process to Denature DNA- Definition, Importance, Techniques and Applications Read More »
qPCR acronym as quantitative PCR is a technique to measure the amount of DNA present in a sample. It can study gene expression by utilizing either the TaqMan probe or
Easiest qPCR Protocol using TaqMan Probe and SYBR Green Dye Read More »
