PCR stands for Polymerase chain reaction. It is a laboratory genetic technique used to amplify DNA and generate copies for downstream analysis. Learn PCR, its principle, steps, advanced protocols, troubleshooting, types and other related information in this category.
Explore all articles on PCR technology and master this essential molecular skill.
Do you know a typical NGS machine can generate around 1.8 to 6TB of sequencing data in a single run? PCR duplication is a serious problem in so many crucial […]
Emulsion PCR: Principle, Process, Advantages, Applications and Limitations Read More »
“False positive and false negative results in PCR are two critical problems. In this article, learn about these two common PCR errors, how it occurs, and how to overcome them.”
PCR Troubleshooting 104: Understanding False Positive and False Negative PCR Results Read More »
“Primer dimers are unintended short amplification products formed in the PCR by unhealthy PCR practices. This article covers common causes for primer dimer formation and troubleshooting.” In the previous article
PCR Troubleshooting 103: How to Address Primer-Dimers Read More »
“Allelic dropout in the PCR is a condition in which one or both the alleles in the heterozygous condition fail to amplify and cause a fault in results. In this
PCR Troubleshooting 102: How to Address The Allelic Dropout Read More »
“Non-specific amplification is one of the most common problems in any PCR reaction. In this guide, I will explain the reasons and possible (technical) solutions to overcome non-specific amplification.” Imagine,
PCR Troubleshooting 101: How to Address Non-Specific Amplification? Read More »
“Sanger sequencing is a genetic technique to determine the order of nucleotides while PCR generates copies of the DNA fragment, present in the sample. Let’s see some of the significant
Sanger Sequencing vs PCR: Common and Technical Differences Read More »
