The annealing temperature is the temperature at which the PCR primers bind to the complementary template region, usually is between 50ºC to 68ºC. We have started exploring PCR, PCR optimization …
What is Annealing Temperature in PCR? How to Calculate and Set It? Read More »
Usually, 25 to 35 cycles a typical PCR reaction needs to get a good amount of amplification, containing denaturation, annealing and extension simultaneously at 95ºC, 50 to 68ºC and 72ºC. …
“A Higher Ct value indicates a lower viral load whilst the lower Ct value indicates a higher amount of viral load in the sample.” I went twice for RT-PCR in …
What is the Ct value of SARS-CoV-2 (COVID-19) RT-PCR? Read More »
“The Ct value is a value used to quantify the absolute amount of nucleic acid present in a sample. It is the number of cycles from where fluorescence increases significantly.” …
Explaining the Ct value (Threshold Cycle) of qPCR Read More »
MLP- Multiplex ligation-dependent Probe AMplification is a nucleic acid quantification technique used in the detection of CNV, SNPs, DNA methylation and mRNA quantification. It is an amplification-based molecular genetic technique. …
MLPA- Multiplex Ligation-dependent Probe Amplification- Process and Advantages Read More »
“PCR- the polymerase chain reaction is one of the most versatile and state of the art techniques used to amplify and quantify DNA.” The PCR machine known as a thermocycler …
