Optimizing PCR Cycles to Get Good Results
Usually, 25 to 35 cycles a typical PCR reaction needs to get a good amount of amplification, containing denaturation, annealing and extension simultaneously at 95ºC, 50 to 68ºC and 72ºC. …
PCR technology
What is the Ct value of SARS-CoV-2 (COVID-19) RT-PCR?
“A Higher Ct value indicates a lower viral load whilst the lower Ct value indicates a higher amount of viral load in the sample.” I went twice for RT-PCR in …
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Explaining the Ct value (Threshold Cycle) of qPCR
“The Ct value is a value used to quantify the absolute amount of nucleic acid present in a sample. It is the number of cycles from where fluorescence increases significantly.” …
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MLPA- Multiplex Ligation-dependent Probe Amplification- Process and Advantages
MLP- Multiplex ligation-dependent Probe AMplification is a nucleic acid quantification technique used in the detection of CNV, SNPs, DNA methylation and mRNA quantification. It is an amplification-based molecular genetic technique. …
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Differences between PCR vs qPCR
“PCR- the polymerase chain reaction is one of the most versatile and state of the art techniques used to amplify and quantify DNA.” The PCR machine known as a thermocycler …
16S rRNA: Gene, Sequencing and Importance
“The 16S rRNA gene is a bacterial ribosomal gene and a part of the 30S subunit which is used in the identification, characterization, and classification of various bacteria.” 16S rRNA …
