What is a Hot-Start PCR?
“Hot start PCR is a modification of conventional PCR in which limiting one of the reagents until the heating step reduces the chances of non-specific amplification.” Non-specific binding or non-specific …
PCR technology
Multiplex PCR- Principle, Process, Protocol, Advantages, Limitations and Applications
“Multiplex PCR amplifies multiple DNA template regions, simultaneously using different sets of primers in a single PCR reaction.” PCR technology has the best utility in research as well as in …
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ARMS or Allele-Specific PCR- Principle, Procedure, Protocol, Applications and Limitations
“A method used to detect a single base change or SNP using sequence-specific primers is referred to as ARMS or Allele-specific PCR.” The PCR technique can detect mutations like deletion, …
What is Immuno-PCR or IPCR?
Immuno PCR/IPCR/qIPCR is a “combination of the efficiency of RT-PCR and specificity of the ELISA method”. As the name suggests the method is a combination of immunology and genetics. The …
PCR Test: 10 tips on how to do it
“The PCR test is performed to get millions of copies of template DNA for performing different genetic applications using the heat dependent rapid amplification method.” Why we always give importance …
What are the Different Components used in the PCR Reaction Buffer?
“The PCR reaction buffer is enriched with the PCR enhancers that increase the overall reaction performance, some of them are Tris, EDTA, MgCl2, KCl, Formamide, DMSO, TritonX100, Nonidet P40, twin20, …
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