“Real-time PCR is a technique used for the quantification of nucleic acid present in the sample using either fluorescence dye or probe-based technique.” PCR is capable enough to amplify the […]
Real-time PCR: Principle, Procedure, Advantages, Limitations and Applications Read More »
“Inverse PCR is used for site-directed mutagenesis and insert studies. Learn about the technique, concept, protocol and applications of inverse PCR.” PCR (Polymerase Chain Reaction) is a technique to amplify
Inverse PCR: Principle, Procedure, Protocol and Applications Read More »
“PCR inhibitors are biological, chemical or other agents that negatively affect the PCR reaction. In this article, learn about various types of PCR inhibitors, the mechanism of inhibition, and how
What are PCR Inhibitors and How to Remove Them? Read More »
“If you know the process of sequencing, amplification by PCR is a dedicated step. Though PCR has its own importance, it is utilized in sequencing for various purposes. Let’s find
Why is PCR used in the Process of DNA Sequencing? Read More »
“Long-range PCR is a method to amplify the DNA fragment of more than 5Kb using a special type of high-fidelity DNA polymerase.” Using the PCR ingredients like dNTPs, PCR buffer,
What is a Long-Range PCR? Read More »
“A rapid colony screening method, the colony PCR validates the target DNA or gene construct present in a bacteria that has grown on selective media.“ As a result, researchers determine
Colony PCR- Principle, Process, Protocol, Advantages and Limitations Read More »
