How to Increase DNA Purity and Yield?
“A pure & good quality DNA has a 260/280 ratio between 1.7 to 1.8 and a yield between 100ng/µL to 300ng/µL is used for PCR, DNA sequencing and other downstream […]
What is DNase?- Definition, Structure, Function and Types
“DNase is the group of DNA enzymes and a type of nuclease used for hydrolytic cleaving of the DNA. It has lucrative applications in biological science and therefore has great
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The Concept of ChIP-Seq (ChIP-Sequencing) Explained
“Sequence information of DNA linked with associated proteins like histones can be obtained by performing high throughput DNA sequencing, such technique is known as ChIP-Seq.” Highlights: ChIP-seq was developed by
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What is Heterochromatin?- Constitutive and Facultative Heterochromatin Explained
“It is believed that the heterochromatin is transcriptionally inactive sequences, and are further classified into constitutive heterochromatin and facultative heterochromatin.” Highlight: Do you know some heterochromatin are still active and
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Factor Affecting DNA Agarose Gel Electrophoresis Results
“Common factors affecting the results of DNA agarose gel electrophoresis are electrical field, nucleic acid sample, buffer and other chemicals used which inversely influence the final results.” Do you know?
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Explaining the Ct value (Threshold Cycle) of qPCR
“The Ct value is a value used to quantify the absolute amount of nucleic acid present in a sample. It is the number of cycles from where fluorescence increases significantly.”
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