What is CIRSPRa or CRISPR activation?
CRISPRi or CRISPR activation is a process to upregulate gene expression or activate genes by effector activators, dcas9 and sgRNA. In 2021, CRISPR have deep penetration in diverse scientific fields. […]
10 Strategies to Achieve Excellent PCR Amplification
“PCR amplification is an enzyme-dependent process to copy a DNA sequence, DNA fragment, genomic DNA, plasmid DNA or any nucleic acid.“ In other words, we can say, Producing multiple copies
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What is CRISPRi or CRISPR interference?
CRISPRi, short for CRISPR interference, is designed to repress gene expression using the customized sgRNA and dCAS. CRISPR- CAS9 is simply amazing! Why? Because it can edit a gene at
dCAS9 (Dead CAS system): Concept, Functions and Applications
“dCAS9 is a deactivated or dead Cas protein that lacks nucleophilic activity or simply is a deactivated nuclease CAS9.” Read more: CRISPR-Cpf1(Cas12a) Gene-Editing System- Explained. You may wonder, Without nucleophilic
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Applications of CRISPR-CAS9 in Medical Science, Diagnostics, Research, Plant Biology and Agriculture
“CRISPR CAS9 is a revolutionary recombinant/ gene-editing technique used in medical research, disease diagnosis, plant research and agriculture.” The era of recombinant DNA technology or more precisely we can say
Process to Denature DNA- Definition, Importance, Techniques and Applications
“DNA denaturation is a process to break the double-stranded DNA into single-stranded by either physical or chemical agents is known as DNA denaturation.” For the last couple of weeks, we
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