What is a Long-Range PCR?
“Long-range PCR is a method to amplify the DNA fragment of more than 5Kb using a special type of high-fidelity DNA polymerase.” Using the PCR ingredients like dNTPs, PCR buffer, […]
What is a Long-Range PCR? Read More »
Colony PCR- Principle, Process, Protocol, Advantages and Limitations
“A rapid colony screening method, the colony PCR validates the target DNA or gene construct present in a bacteria that has grown on selective media.“ As a result, researchers determine
Colony PCR- Principle, Process, Protocol, Advantages and Limitations Read More »
What is an In Silico PCR? How to Perform it?
“An in silico PCR is a computational tool used to determine the results of practical PCR reaction computationally using a set of the selected primers.” Here in the in silico
What is an In Silico PCR? How to Perform it? Read More »
How to set up a DNA extraction lab: A comprehensive guide (chemicals, instruments and other utilities).
Setting up a DNA extraction lab is quite difficult for the novice. However, it is not a big task yet we have to select all the utilities very carefully. which
What is Touchdown (TD)-PCR?
In the touchdown PCR, “By sequentially decreasing the annealing temperature during each PCR cycle, the chance of the non-specific binding can be reduced.” Every PCR technique is evolved to eliminate
What is Touchdown (TD)-PCR? Read More »
Template DNA in PCR: Function, Properties and Concentration
“Template DNA in PCR is a single-stranded DNA that provides a starting point for amplification. Explore the role, properties and concentration of template DNA and learn how to optimize it.”
Template DNA in PCR: Function, Properties and Concentration Read More »