“ddNTPs are known as chain termination nucleotides. In this article, learn about what ddNTPs are, why we are using them, and the difference between ddNTP and dNTP.” PCR and DNA […]
ddNTP in Sanger Sequencing- What Is It and Why We Use It? Read More »
“DNA fragmentation is a technique of artificial breaking of DNA in order to use it in various downstream genetics and well as genomics applications.” The first DNA fragmentation was reported
DNA fragmentation- Techniques, Importance and Applications Read More »
“The mRNA possesses the sequences to encode protein only thus to construct the DNA library or the cDNA library, the mRNA is reverse transcribed into the cDNA and processed.” The
Why is mRNA chosen for making DNA Library instead of Genomic DNA? Read More »
“Determining the nucleotide sequence orders of the whole exome set of an organism using a high throughput DNA sequencing method is known as whole-exome sequencing.” Three different biomolecules construct a
What is Whole-Exome Sequencing? Read More »
“The 16S rRNA gene is a bacterial ribosomal gene and a part of the 30S subunit which is used in the identification, characterization, and classification of various bacteria.” 16S rRNA
16S rRNA: Gene, Sequencing and Importance Read More »
“Steps in preparing DNA for NGS and any other sequencing platforms are DNA isolation, DNA yield and quality check, library preparation, amplification and sequencing. The success of entire experiments highly
How to Prepare DNA For Sequencing or NGS? Read More »
