Gel electrophoresis is a fundamental and subsidiary technique in molecular biology that separates DNA, RNA or proteins based on their charge and size. It enables scientists to study and visualize the results of nucleic acid extraction, PCR, restriction digestion and sequencing. In this category, you will learn the basics of gel electrophoresis, principles, process, protocols, troubleshooting and applications.
Read detailed articles and tutorials on gel electrophoresis here.
“Explore the comparison between Agarose and PAGE gel and find out which is for which. Understand the differences in their composition, working, applications and more in the present article.” DNA […]
Clash of Gels- Agarose Gel vs PAGE Gel Read More »
“Learn about the crucial molecular genetic process- gel DNA isolation in this article. Explore how the process is done, the steps, applications, advantages and importance of isolating DNA from a
How to Extract DNA From a Gel?- A Step-By-Step Guide Read More »
“Common problems in gel electrophoresis are the absence of bands, band smears, weak resolution, diffused bands, wavy bands and poor band separation. This article comprises common troubleshooting to overcome these
Common Issues in DNA/RNA Gel Electrophoresis and Troubleshooting Read More »
EMSA stands for Electrophoretic Mobility Shift Assay often known as gel shift assay is a technique to study the nucleic acid and protein interaction. The present technique is used ‘in
What is Gel Shift Assay or EMSA? Read More »
“To get success in the gel electrophoresis of DNA we need to use a proper concentration of each chemical, an exact concentration of agar, and need to do some routine
10 Proven Tips to Success in Gel Electrophoresis of DNA Read More »
“The DNA ladder is a standard-sized molecular marker or fragments of DNA applied to determine the size of PCR amplicons. 50bp, 100bp, 1000bp and 3000bp are several commercially available and
What is a DNA ladder? and How To Prepare It? (A Complete Protocol) Read More »
